US EPA Manual of Methods for Virology
The electronic text form of the US EPA MANUAL OF METHODS FOR VIROLOGY (EPA/600/4-84/013) is composed of 16 chapters. These chapters are presented here.
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- Chapter 1. Introduction (PDF) (10 pp, 45K)
- Chapter 2. Cleansing Laboratory Ware and Equipment (PDF) (24pp, 219K)
- Chapter 3. Sterilization and Disinfection (PDF) (5 pp, 90K)
- Chapter 4. Quality Assurance (PDF) (19 pp, 108K)
- Chapter 5. Virus Adsorption-Elution (Viradel) Disc Filter Procedures for Recovering Viruses from Sewages, Effluents, and Waters (PDF) (30 pp, 1MB)
- Chapter 6. Virus Adsorption-Elution (Viradel) Cartridge Filter Procedures for Recovering Viruses from Sewage, Effluents, and Waters (PDF) (46 pp, 1.3MB)
- Chapter 7. Method for Recovering Viruses from Sludges (and Other Solids) (PDF) (16 pp, 263K)
- Chapter 8. Method for Reduction of Cytotoxicity of Sample Concentrates (PDF) (9 pp, 48K)
- Chapter 9. Cell Culture Preparation and Maintenance (PDF) (21 pp, 11K)
- Chapter 10. Cell Culture Procedures for Assaying Plaque-Forming Viruses (PDF) (28 pp, 263K)
- Chapter 11. Virus Plaque Confirmation Procedures (PDF) (6 pp, 34K)
- Chapter 12. Identification of Enteroviruses (PDF) (10 pp, 137K)
- Chapter 13. Data Analysis and Experimental Design (not yet available in PDF format)
- Chapter 14. Concentration and Processing of Waterborne Viruses by Positive Charge 1MDS Cartridge Filters and Organic Flocculation (PDF) (12 pp, 1.1MB)
- Chapter 15. Total Culturable Virus Quantal Assay (PDF) (11 pp, 1MB)
- Chapter 16. Procedures for Detecting Coliphages (PDF) (10 pp, 593K)
This Manual is also available in hardcopy form. The hardcopy form of the Manual can be obtained by contacting:
Branch Chief, Biohazard Assessment Research Branch ([email protected])
Microbiological and Chemical Exposure Assessment Research Division
National Exposure Research Laboratory
U.S. Environmental Protection Agency
26 West M.L. King Dr.
Cincinnati OH 45268 USA
Original Date of Publication: February 1984
Subsequent Chapter Revision/Addition Dates: 1986, 1987, 1988, 1989, 1991,
1993 , 2001