Project Number: 1235-51000-046-02
Project Type: Reimbursable

Start Date: Sep 01, 2005
End Date: Aug 31, 2009

Objective:
The purpose of this agreement is to characterize the biological response to dietary exposure to naturally occurring dietary constituents, allyl isothiocyanates (AITC). The proposed studies will compare the availability and metabolism of AITC from various food sources and contrast the response in terms of ability to reduce DNA damage and to monitor genetic and proteomic expression changes resulting from dietary exposure.

Approach:
Human studies will be conducted with subjects consuming naturally occurring constituents found in cruciferous vegetables, namely allyl isothiocyanates. Healthy adults will be assigned to treatments consisting of varying amounts and/or types of allyl isothiocyanate-rich foods (cruciferous vegetables such as Brussels sprouts, cabbage, Wasabi) or commercially available extracts containing allyl isothiocyanates. Intake levels will not be pharmacological, but rather will be limited to levels achievable with commonly consumed amounts of allyl isothiocyanate-containing foods. Subjects will consume the foods for up to 60 days, and biological samples will be collected and analyzed. Availability of parent compounds and their metabolites will be determined. Target outcomes include indices of oxidation and changes in genetic and proteomic expression.