Location: Molecular Characterization of Foodborne Pathogens
Project Number: 1935-42000-067-01
Start Date: Jun 09, 2009
End Date: Sep 30, 2009
Objective:
Approach:
The efficacy of currently available methods and newly devised advantageous methods for screening and quantitation of cyanobacterial toxins in catfish filets and other catfish products will be investigated. Immunochemical (enzyme-linked immunosorbent assays, ELISA) and analytical (liquid chromatography-tandem mass spectrometry, LC-MS/MS) methods will be developed and/or evaluated. Purified cyanobacterial toxins are commercially available and will be purchased for use as reference standards for experiments. Catfish samples will be fortified with known concentration of cyanobacterial toxins for analysis by developed methods. Blank samples will also be tested as controls for comparison purposes.